Plasmodium belongs to the phylum Chromatophora, Phylum Acanthozoa, Class Vertebrata, Order Haemosporidae, and Family Plasmodium. It is a mosquito-borne sporozoite, and is the causative agent of malaria. Plasmodium parasitic in humans has two hosts, mosquitoes and humans, and can reproduce sexually in mosquitoes or proliferate asexually in humans. Anopheles mosquitoes carrying Plasmodium are transmitted by biting humans, causing malaria fever and cold episodes. Other species of Plasmodium infect other species of animals, including other primates, rodents, and lizard-like animals.

Malaria kills nearly one million people worldwide each year, and there is a lack of efficient tools to study the gene function of its causative parasite Plasmodium berghei. CRISPR/Cas9 has been successfully applied to various model species as a novel and efficient genome modification method.

Ace Therapeutics specializes in parasitology and has established a parasite genetic engineering technology platform designed to provide genetic engineering services for a variety of parasitic diseases to clients worldwide. We can provide CRISPR/Cas9 gene editing services for Plasmodium, laying the foundation for in-depth elucidation of mechanistic studies.

CRISPR/Cas9 Genome Editing Service Options in Plasmodium

We established a CRISPR/Cas9 genomic modification method for Plasmodium, which can efficiently perform gene knockout, gene plus tag and nucleotide substitution modifications in Plasmodium. Based on this, the method was further developed to address the technical needs of multiple gene modifications in the study of Plasmodium gene function. It provides a panoramic view to understand the regulation of gene expression in the life cycle of Plasmodium. If you want to know more, you are welcome to consult us.

We can provide CRISPR/Cas9 genomic modification services for different Plasmodium species, including but not limited to the following:

• CRISPR/Cas9 genomic modifications of Plasmodium yoelii in mice
• CRISPR/Cas9 genomic modifications of human Plasmodium falciparum
• CRISPR/Cas9 genomic modification of Plasmodium berghei

Major modification types:

• Gene knockout
• Gene tag insertion
• Gene replacement modification model

Applications

• This has laid the foundation for the construction of transgenic Plasmodium and the establishment of its animal model.
• It also provides a reliable and efficient solution for the screening of anti-Plasmodium drugs and vaccines and the study of Plasmodium.

Service Details

Delivery content: experiment report and Plasmodium or Plasmodium animal models that edited by CRISPR/Cas9 technology
Test fee: please get it through online inquiry.

Service Flow

1. Communicate your requirements with us via online inquiry or telephone.
2. Communicate the requirements, sign the contract and pay the deposit.
3. Fill out the sample order and send the samples (optional).
4. Establish the needed Plasmodium CRISPR/Cas9 system and prepare experiment report. (All steps from vector design, construction, activity identification, and establishment of genetically mutated Plasmodium or model are completed in a one-stop shop.)
5. Delivery of report and Plasmodium edited by CRISPR/Cas9 technology.

Our Advantages

• We have already had many years of successful experience in CRISPR/Cas9 system construction and activity validation.
• We have a strong technical support team and can provide free professional technical support for our customers.
• We have successfully used CRISPR/Cas9 system to provide thousands of successful cases for our customers. Our service is unanimously recognized by our customers.
• Low price and short cycle time.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service