In recent years, the rapid development of protein microarrays has greatly promoted and facilitated a number of studies in protein diagnostics and proteomics. Currently, protein microarrays are mainly used for immunoassay, enzyme activity determination, expression screening, drug target study, and disease diagnosis. Given that protein microarrays have the characteristics of high throughput, high efficiency, rapidity, a small number of samples, and easy operation, the application of this technology will become more widespread. Its application can play an important role in parasitological research, such as for gene expression screening, antigen-antibody detection, biochemical reaction detection, and anti-parasitic vaccine target screening and drug development.

Ace Therapeutics provides technical services of protein microarray systems for parasitic organisms to customers worldwide and has established a comprehensive protein microarray service system that can widely cover most of the scientific needs related to parasitic microorganisms such as signaling pathways, cytokines, protein expression, protein modifications, protein interactions, disease marker screening, etc., for you to choose flexibly according to your needs.

Protein Microarray Development Service Options for Parasitic Organisms

We can also provide protein microarray technology services for a wide range of parasitic organisms, including but not limited to animals, microorganisms, protozoa, fungi, viruses, etc.

• Animals — roundworms, tapeworms, pinworms, schistosomes, fleas, lice, etc.
• Microorganisms — E. coli, dysentery bacilli, etc.
• Protozoa — amoebae within dysentery, malaria parasites, etc.
• Fungi — ringworm of the hands, ringworm of the skin, etc.
• Parasitic edible bacteria — Cordyceps, honeysuckle, etc.
• Virus — parasitic, such as adenovirus, prion, etc.

Note: In addition, there are other technical services available for you to choose from, please consult us for details.

Experimental Operation Process

1. Customer provides parasitic organism sample (tissue /cell /RNA /others)
2. Microarray printing.
3. Chip fixation. If there is no follow-up experiment, the chip is delivered to the customer.
4. Microarray hybridization reaction, (antibody/antigen labeling)
5. Microarray scanning and data analysis. The chip is cleaned and scanned, and data is extracted for analysis.
6. Provide the result report (including hybridization pictures, data results, and corresponding results of clustering and difference analysis, etc.).

Fig. 1 Parasite protein microarray workflow

Customer Provides

• A fresh parasite tissue sample (50-100 mg), or extracted RNA.
• Cell samples of parasitic organisms (1×10⁶ -1x10⁷ cells), or extracted RNA.
• For other samples, please consult us for details.

Delivery Standard

• Protein microarray of the parasite
• Microarray detection data
• Complete experimental protocols, experimental reports (including software analysis results), and experimental materials

Technical Advantages

• High throughput (it enables parallel analysis of thousands of proteins.)
• Micronization (minimal sample volume required)
• Relatively simple to use and high correctness of results
• Simple operation and easy analysis
• Short experimental cycle time

Reference

1. Peacock, Christopher. [Methods in Molecular Biology] Parasite Genomics Protocols Volume 1201 || Protein Microarrays for Parasite Antigen Discovery[J]. 2015, 10.1007/978-1-4939-1438-8(Chapter 13):221-233.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service