Cryptosporidiosis is a zoonotic intestinal parasitic disease caused by infection with protozoa of the genus Cryptosporidium in the subphylum Terminalia (protozoa). The lesions mainly injure the mucosa of the small intestine, and the clinical manifestations are mainly watery diarrhea, often with upper abdominal discomfort, nausea and vomiting. Immunocompetent adult patients are self-limiting, while immunodeficient or malnourished children require anti-Cryptosporidium treatment. The pathogenesis of Cryptosporidium is uncertain.

Transfection is a technique that introduces exogenous genes into eukaryotic cells and expresses them intracellularly. Using transfection technology to construct Cryptosporidium transfection vectors will provide an effective tool to study the function of Cryptosporidium genes and to screen drug target genes and protective antigen genes.

Ace Therapeutics specializes in parasitology and has established a parasite genetic engineering technology platform designed to provide genetic engineering services for a variety of parasitic diseases to clients worldwide. We are able to provide transfection services for Cryptosporidium to our global clients, laying the foundation for in-depth elucidation of mechanistic studies.

Gene Transfection Service Options for Cryptosporidium

We have established the Cryptosporidium transfection system, mainly by electroporation transformation method. Our Cryptosporidium transfection service is still undergoing condition optimization and improvement. If you need to know more, please consult us for details.

Transfection of mouse intestinal epithelial cells (IEC) (liposome transfection method)

• Isolation and culture of mouse intestinal epithelial cells (IEC).
• To establish a primary culture method for mouse IEC cells and to obtain an in vitro model of intestinal epithelial cells parasitized by Cryptosporidium.
• The recombinant eukaryotic plasmid was introduced into mouse IEC cells for expression using liposome transfection method.

Application of Cryptosporidium gene transfection

• Study of Cryptosporidium gene expression regulation
• Study of Cryptosporidium gene function
• Cryptosporidium drug research
• Cryptosporidium vaccine research
• Cell biology research

Service Details

Delivery content: experiment report and products of Cryptosporidium gene transfection
Test fee: please get it through online inquiry.

Service Flow

1. Communicate your requirements with us via online inquiry or telephone.
2. Client sends experiment plan.
3. Communicate service strategy, sign contract and pay deposit.
4. Perform the service of Cryptosporidium gene transfection and prepare the experiment report. (One-stop service for vector construction, optimization of transformation conditions by electroporation, transfectant identification, etc.)
5. Deliver the report and the product of Cryptosporidium gene transfection.

Our Advantages

• We have many years of successful experience in parasite genetic engineering services.
• We have improved transfection methods continuously.
• Professional technical platform, scientific management system and standard operation plan.
• We have a strong technical support team and can provide free professional technical support to our customers.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service